Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Others

Cell type

Fibroblasts

NA

NA

Sample

source_name

Fibroblast cell line, PAX3-FOXO1 vector added

cell line

7250

cell type

fibroblast

antibody

NA

enrichment target

DNase-sensitivity

target function

Open Chromatin

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

25311339

Reads aligned (%)

86.8

Duplicates removed (%)

45.7

Number of peaks

5030 (qval < 1E-05)

hg19

Number of total reads

25311339

Reads aligned (%)

86.0

Duplicates removed (%)

46.2

Number of peaks

4549 (qval < 1E-05)