Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Muscle

Cell type

RMS209

NA

NA

Sample

source_name

RMS primary tumor, PAX3-FOXO1, MYCN amp

cell line

RMS209

cell type

rhabdomyosarcoma

antibody

H3K27ac (Active Motif, 39133)

enrichment target

H3K27ac

target function

Active enhancers

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. DNase DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

28213330

Reads aligned (%)

96.9

Duplicates removed (%)

60.6

Number of peaks

10097 (qval < 1E-05)

hg19

Number of total reads

28213330

Reads aligned (%)

96.5

Duplicates removed (%)

61.1

Number of peaks

9991 (qval < 1E-05)