Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2A

Cell type

Cell type Class
Blood
Cell type
ML-2
Primary Tissue
Bone Marrow
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Leukemia cell line (ML-2)
cell line
Leukemia cell line; ML-2
cell type
Adult monocytic cell line derived from AML M4 with t(6;11)(q27;q23) translocation
chip antibody
MLL1 (Bethyl, A300-086A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Nascent RNA-seq: 108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040) or Ultra RNA Library Prep Master Mix Set (Part# E7530).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16519117
Reads aligned (%)
185.3
Duplicates removed (%)
5.0
Number of peaks
459 (qval < 1E-05)

hg38

Number of total reads
16519117
Reads aligned (%)
188.0
Duplicates removed (%)
4.8
Number of peaks
515 (qval < 1E-05)

Base call quality data from DBCLS SRA