Antigen

Antigen Class

TFs and others

Antigen

KMT2A

Cell type

Cell type Class

Blood

Cell type

ML-2

Primary Tissue

Bone Marrow

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Leukemia cell line (ML-2)

cell line

Leukemia cell line; ML-2

cell type

Adult monocytic cell line derived from AML M4 with t(6;11)(q27;q23) translocation

chip antibody

MLL1 (Bethyl, A300-086A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Nascent RNA-seq: 108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040) or Ultra RNA Library Prep Master Mix Set (Part# E7530).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

16519117

Reads aligned (%)

98.8

Duplicates removed (%)

4.8

Number of peaks

515 (qval < 1E-05)

hg19

Number of total reads

16519117

Reads aligned (%)

97.6

Duplicates removed (%)

5.0

Number of peaks

459 (qval < 1E-05)