Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MDM2

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT116
cell line
HCT116
chip antibody
MDM2
treatment
Untreated
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6836716
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates were sonicated resulting in an average chromatin fragment size of ~300 bp and immunoprecipiated with the indicated antibodies. Libraries were prepared by ligating Illumina TrueSeq adapters adpators followed by 14 cycles of amplification

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
17462659
Reads aligned (%)
96.9
Duplicates removed (%)
4.0
Number of peaks
692 (qval < 1E-05)

hg19

Number of total reads
17462659
Reads aligned (%)
95.9
Duplicates removed (%)
4.2
Number of peaks
530 (qval < 1E-05)

Base call quality data from DBCLS SRA