Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)

Attributes by original data submitter

Sample

source_name
MEL
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
MEL
cell organism
mouse
cell description
Leukemia (K562 analog)
cell sex
M
antibody
H3K9me3
antibody antibodydescription
rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3
antibody targetdescription
Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.
antibody vendorname
Abcam
antibody vendorid
ab8898
treatment
DMSO_2.0pct
treatment description
5 d with 2% Dimethyl sufloxide (DMSO) (Weissman)
control
std
control description
Standard input signal for most experiments.
age
immortalized
age description
Immortal cells
control
std
control description
Standard input signal for most experiments.
controlid
MEL/DMSO_2.0pct/Input/std
strain
Unknown
strain description
Unknown strain origin

Sequenced DNA Library

library_name
GSM1003748: Stanford_ChipSeq_MEL_H3K9me3_DMSO_2.0pct_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
61634319
Reads aligned (%)
96.8
Duplicates removed (%)
24.0
Number of peaks
692 (qval < 1E-05)

mm9

Number of total reads
61634319
Reads aligned (%)
96.5
Duplicates removed (%)
24.0
Number of peaks
799 (qval < 1E-05)

Base call quality data from DBCLS SRA