Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit Polyclonal Antigen Affinity Purified, Unconjugated, Liquid
antibody targetdescription
A transcriptional repressor which represses neuronal genes in non-neuronal tissues. A member of the Kruppel-type zinc finger transcription factor family. Represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE) by associating with two corepressors, mSin3 and CoREST, which recruit HDACs to the promoters of REST-regulated genes. Target is GST fusion amino acids 801-1097 of human REST.
antibody vendorname
Millipore
antibody vendorid
17-641
controlid
wgEncodeEH000052
replicate
1
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
REST
antibody antibodydescription
Rabbit Polyclonal Antigen Affinity Purified, Unconjugated, Liquid
antibody targetdescription
A transcriptional repressor which represses neuronal genes in non-neuronal tissues. A member of the Kruppel-type zinc finger transcription factor family. Represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE) by associating with two corepressors, mSin3 and CoREST, which recruit HDACs to the promoters of REST-regulated genes. Target is GST fusion amino acids 801-1097 of human REST.
antibody vendorname
Millipore
antibody vendorid
17-641
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
K562/None/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003507: Broad_ChipSeq_K562_REST
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
40751189
Reads aligned (%)
27.8
Duplicates removed (%)
70.6
Number of peaks
4612 (qval < 1E-05)

hg38

Number of total reads
40751189
Reads aligned (%)
30.0
Duplicates removed (%)
68.6
Number of peaks
4601 (qval < 1E-05)

Base call quality data from DBCLS SRA