Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
biomaterial_type
immortalized cell line
line
HeLa-S3
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3
antibody targetdescription
Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.
antibody vendorname
abcam
antibody vendorid
ab8898
controlid
wgEncodeEH001013
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
H3K9me3
antibody antibodydescription
rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3
antibody targetdescription
Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.
antibody vendorname
Abcam
antibody vendorid
ab8898
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
HeLa-S3/Input/std
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003480: Broad_ChipSeq_HeLa-S3_H3K9me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
63935455
Reads aligned (%)
97.4
Duplicates removed (%)
17.0
Number of peaks
2660 (qval < 1E-05)

hg19

Number of total reads
63935455
Reads aligned (%)
95.9
Duplicates removed (%)
19.6
Number of peaks
1990 (qval < 1E-05)

Base call quality data from DBCLS SRA