Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Blood
Cell type
CD20+
Tissue
blood
Lineage
mesoderm
Description
B cells from donors RO01778 and RO01794, newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
CD20+
biomaterial_provider
Dr. Heimfeld Lab at FHCRC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal. Antibody Target: CTCF
antibody targetdescription
CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.
antibody vendorname
Millipore
antibody vendorid
07-729
controlid
wgEncodeEH003132
replicate
1,3,2
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
CTCF
antibody antibodydescription
rabbit polyclonal. Antibody Target: CTCF
antibody targetdescription
CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.
antibody vendorname
Millipore
antibody vendorid
07-729
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
CD20+/None/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003474: Broad_ChipSeq_CD20+_CTCF
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
91297618
Reads aligned (%)
91.7
Duplicates removed (%)
47.0
Number of peaks
38694 (qval < 1E-05)

hg19

Number of total reads
91297618
Reads aligned (%)
91.0
Duplicates removed (%)
48.2
Number of peaks
38387 (qval < 1E-05)

Base call quality data from DBCLS SRA