Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Bone

Cell type

Osteobl

Tissue

bone

Lineage

mesoderm

Description

osteoblasts (NHOst)

Sample

source_name

Osteobl

biomaterial_provider

Lonza

donor_id

ENCDO834SRG,ENCDO271AAA

biomaterial_type

primary cell

cell_type

osteoblast

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27me3

antibody targetdescription

Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.

antibody vendorname

Millipore

antibody vendorid

07-449

controlid

wgEncodeEH001056

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

U

antibody

H3K27me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27me3

antibody targetdescription

Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.

antibody vendorname

Millipore

antibody vendorid

07-449

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

Osteobl/Input/std

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003466: Broad_ChipSeq_Osteobl_H3K27me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

50535332

Reads aligned (%)

94.5

Duplicates removed (%)

17.5

Number of peaks

532 (qval < 1E-05)

hg19

Number of total reads

50535332

Reads aligned (%)

94.0

Duplicates removed (%)

18.3

Number of peaks

482 (qval < 1E-05)