Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549

biomaterial_provider

ATCC

biomaterial_type

immortalized cell line

line

A549

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K36me3

antibody targetdescription

Specific for histone H3 tri methylated at lysine 36, weakly reacts with H3K36me2. Marks regions of RNAPII elongation, including coding and non-coding transcripts.

antibody vendorname

abcam

antibody vendorid

ab9050

controlid

wgEncodeEH003076

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

M

antibody

H3K36me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K36me3

antibody targetdescription

Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.

antibody vendorname

Abcam

antibody vendorid

ab9050

treatment

EtOH_0.02pct

treatment description

1 h with 0.02% Ethanol (Myers)

control

std

control description

Standard input signal for most experiments.

controlid

A549/EtOH_0.02pct/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003456: Broad_ChipSeq_A549_H3K36me3_EtOH_0.02pct

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

165515716

Reads aligned (%)

96.2

Duplicates removed (%)

10.6

Number of peaks

7198 (qval < 1E-05)

hg19

Number of total reads

165515716

Reads aligned (%)

95.3

Duplicates removed (%)

12.7

Number of peaks

6891 (qval < 1E-05)