Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Neural
Cell type
SK-N-SH
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
SK-N-SH
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
SK-N-SH
cell organism
human
cell description
neuroblastoma, newly promoted to tier 2: not in 2011 analysis, the SK-N-SH line was established from a neuroblastoma in a 4 year old individual.
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
p300_(SC-584)
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the N-terminus of p300 of human origin. Antibody Target: P300
antibody targetdescription
Encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-584
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH003393

Sequenced DNA Library

library_name
GSM1003626: Stanford_ChipSeq_SK-N-SH_p300_(SC-584)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
64244108
Reads aligned (%)
86.1
Duplicates removed (%)
9.9
Number of peaks
55376 (qval < 1E-05)

hg38

Number of total reads
64244108
Reads aligned (%)
87.6
Duplicates removed (%)
8.8
Number of peaks
55561 (qval < 1E-05)

Base call quality data from DBCLS SRA