Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testis

biomaterial_provider

1)LICR lab; 2)CSHL lab

lab

LICR-m

lab description

Ren - Ludwig Institue for Cancer Research

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

Testis

cell organism

mouse

cell description

Testis

cell sex

M

antibody

H3K27me3

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27me3

antibody targetdescription

Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.

antibody vendorname

Millipore

antibody vendorid

07-449

age

adult-8wks

age description

Adult 8 weeks

age

adult-8wks

age description

Adult 8 weeks

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEM002490

labversion

Pooled & filtered

softwareversion

MACS

strain

C57BL/6

strain description

C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name

GSM1000145: LICR_ChipSeq_Testis_H3K27me3_adult-8wks

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

84664791

Reads aligned (%)

84.4

Duplicates removed (%)

35.5

Number of peaks

893 (qval < 1E-05)

mm9

Number of total reads

84664791

Reads aligned (%)

84.1

Duplicates removed (%)

35.4

Number of peaks

1120 (qval < 1E-05)