Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
BMDM
biomaterial_provider
Genhong Cheng Lab, UCLA
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
BMDM
cell organism
mouse
cell description
Bone marrow derived macrophage
cell sex
F
antibody
H3K27ac
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription
Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname
Abcam
antibody vendorid
ab4729
age
adult-8wks
age description
Adult 8 weeks
age
adult-8wks
age description
Adult 8 weeks
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEM002660
labversion
Pooled & filtered
softwareversion
MACS
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM1000074: LICR_ChipSeq_BMDM_H3K27ac_adult-8wks
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
27571897
Reads aligned (%)
91.3
Duplicates removed (%)
17.0
Number of peaks
49174 (qval < 1E-05)

mm9

Number of total reads
27571897
Reads aligned (%)
91.2
Duplicates removed (%)
17.1
Number of peaks
49253 (qval < 1E-05)

Base call quality data from DBCLS SRA