ChIP-Atlas: SRX18517800

Antigen

Cell type Class: Epidermis
Cell type: Keratinocytes
MeSH Description: Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: RNA was extracted and DNase treated using the Quick-RNA Miniprep Kit by Zymo Research. For ChIP-seq samples, cells were crosslinked with 1% formaldehyde for 10 minutes and then quenched with glycine. Nuclei were extracted in nuclei extraction buffer (10 mM HEPES pH7.4, 1.5 mM MgCl2, 10 mM KCl, and 0.2% NP-40) and lysed using a 27(1/2) gauge needle. DNA in nuclei lysate was fragmented to ~200 bp using sonication. Sonicated lysated was incubated with protein G beads conjugated with antibody. Beads were washed and DNA was eluted. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina was used to prepare RNA-seq libraries. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina was used to prepare ChIP-seq libraries.