Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gcn5

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cells
treatment
GFP dsRNA treated S2 cells
antibody
Gcn5 rabbit polyclonal antibody (5 ug/IP)
cell type
S2 cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Kc cells were washed with PBS and croslinked with formaldehyde (1%). Formaldehyde was quenched with glycine. Cells were washed twice with ice-cold PBS. Nuclei were exrtacted with IP buffer . Nuclei were sheared and incubated with antibody overnight. immunocomplexes were collected by agarose A/G beads. DNA was purified with Chelex-100 beads. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
21414894
Reads aligned (%)
94.3
Duplicates removed (%)
51.8
Number of peaks
11327 (qval < 1E-05)

dm6

Number of total reads
21414894
Reads aligned (%)
94.0
Duplicates removed (%)
53.6
Number of peaks
10980 (qval < 1E-05)

Base call quality data from DBCLS SRA