Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
Kc167

Cell type information


Source
e/se
Developmental Stage
dorsal closure stage

Attributes by Original Data Submitter


source_name
Kc cells
treatment
GFP dsRNA treated Kc cells
antibody
none
cell type
Kc cells

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Kc cells were washed with PBS and croslinked with formaldehyde (1%). Formaldehyde was quenched with glycine. Cells were washed twice with ice-cold PBS. Nuclei were exrtacted with IP buffer . Nuclei were sheared and incubated with antibody overnight. immunocomplexes were collected by agarose A/G beads. DNA was purified with Chelex-100 beads. Libraries were prepared according to Illumina's instructions

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
13421313
Reads aligned (%)
87.9
Duplicates removed (%)
48.7
Number of peaks
2825 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA