GSM2202470: Gcn5 ChIP-Seq in Kc cells treated with CP190 RNAi; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Gcn5
Cell type
Cell type Class
Cell line
Cell type
Kc167
Source
e/se
Developmental Stage
dorsal closure stage
Attributes by original data submitter
Sample
source_name
Kc cells
treatment
CP190 dsRNA treated Kc cells
antibody
Gcn5 rabbit polyclonal antibody (5 ug/IP)
cell type
Kc cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Kc cells were washed with PBS and croslinked with formaldehyde (1%). Formaldehyde was quenched with glycine. Cells were washed twice with ice-cold PBS. Nuclei were exrtacted with IP buffer . Nuclei were sheared and incubated with antibody overnight. immunocomplexes were collected by agarose A/G beads. DNA was purified with Chelex-100 beads. Libraries were prepared according to Illumina's instructions