Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

Enterocytes

MeSH Description

Absorptive cells in the lining of the INTESTINAL MUCOSA. They are differentiated EPITHELIAL CELLS with apical MICROVILLI facing the intestinal lumen. Enterocytes are more abundant in the SMALL INTESTINE than in the LARGE INTESTINE. Their microvilli greatly increase the luminal surface area of the cell by 14- to 40 fold.

Sample

source_name

mouse enterocyte progenitor cells

strain

B6; 129 mixed background

cell type

Adult Epitheial cells; proximal 1/3 of intestine

genotype

Atoh1 F/F; Villin-CRE

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysates were sonicated and incubated with 2 ug H3K4me1 antibody overnight (C15410194, Diagenode) for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

35250216

Reads aligned (%)

94.0

Duplicates removed (%)

5.6

Number of peaks

52116 (qval < 1E-05)

mm9

Number of total reads

35250216

Reads aligned (%)

93.9

Duplicates removed (%)

5.7

Number of peaks

52121 (qval < 1E-05)