90m stimulation with 15% serum media + cycloheximide
passage
3
chip antibody
Abcam ab4729 lot GR150367-1
cell line
Primary fibroblasts
serum stimulation
90m
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Growth media was removed and MEFs were crosslinked in 1% formaldehyde for 10 minutes at room temperature before quenching with glycine at a final concentration of 0.125M. Sonicated chromatin from isolated nuclei were used as input to ChIP. 2-40 ng of each ChIP sample was used to prepare ChIP-Seq libraries with the NuGEN Ovation Ultralow Library System v2 kit.