Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Neural

Cell type

Brain

MeSH Description

The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.

Sample

tissue

forebrain

age

0 day

donor ID

ENCDO469XMJ

dev stage

postnatal

sample type

tissue

sex

pooled male and female

health state

healthy

lab

Len Pennacchio, LBNL

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

document: https://www.encodeproject.org/documents/89795b31-e65a-42ca-9d7b-d75196f6f4b3/@@download/attachment/Ren_Chromatin_Immunoprecipitation.pdf document: https://www.encodeproject.org/documents/4f73fbc3-956e-47ae-aa2d-41a7df552c81/@@download/attachment/Ren_ChIP_Library_Preparation_v060614.pdf document: https://www.encodeproject.org/documents/3125496b-c833-4414-bf5f-84dd633eb30d/@@download/attachment/Ren_Tissue_Fixation_and_Sonication_v060614.pdf See ENCODE portal

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

36752283

Reads aligned (%)

98.1

Duplicates removed (%)

20.4

Number of peaks

561 (qval < 1E-05)

mm9

Number of total reads

36752283

Reads aligned (%)

98.0

Duplicates removed (%)

20.5

Number of peaks

616 (qval < 1E-05)