GSM2193215: Input WT 3xFLAG WT ZNF335; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
Jurkat cells
cell line
Jurkat T-REx
protein expression
3xFLAG WT ZNF335
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fragmented by a combination of sonication and MNase digestion. Chromatin immunoprecipitation was performed using anti-FLAG M2 bound to Protein G Dynabeads. Beads were washed in low salt buffer, high salt buffer, LiCl buffer and TE buffer. Protein/DNA complexes were eluted, reverse-crosslinked overnight at 65°C and treated with RNase A and proteinase K. ChIP DNA was purified using Qiaquick PCR Purification kit (Qiagen). Approximately 150 ng DNA was used for library construction according to Illumina’s TruSeq protocol. Libraries were size-selected (200–500 bp) by agarose gel purification, assessed for quality using the High Sensitivity DNA Bioanalyzer kit (Agilent). Libraries were sequenced on the Illumina HiSeq 2000 platform as 50 bp single-end reads