Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA1

Cell type

Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
CWR22-Rv1
cell line
CWR22-Rv1
cell type
Prostate cancer cell line
chip antibody
anti-FOXA1
treatment
Veh
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM6734607
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde and then sonicated. DNA were immunoprecipitatted with antibody and protein A/G beads. After reverse crosslinking, DNA were purifed with Qiagen PCR purification kit. Libraries were constructed using the SMARTer ThruPLEX DNA-Seq Prep Kit (Takara Bio USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
26454716
Reads aligned (%)
93.2
Duplicates removed (%)
7.0
Number of peaks
23535 (qval < 1E-05)

hg19

Number of total reads
26454716
Reads aligned (%)
92.6
Duplicates removed (%)
8.1
Number of peaks
23541 (qval < 1E-05)

Base call quality data from DBCLS SRA