Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUND

Cell type

Cell type Class
Bone
Cell type
Osteoblasts
MeSH Description
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.

Attributes by original data submitter

Sample

source_name
DIF_JUND
immortalization type
SV40 large T antigen immortalized
cell type
human Fetal Osteoblasts (hFOB)
protocol
differentiated for 5 days into osteoblastic lineage
chip antibody
Santa Cruz, sc-74,329

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIP was performed with 20 min crosslinking in 1 % formaldehyde in PBS. Following chromatin shearing , 1-2µg antibody was used for immunoprecipitation overnight. The immunocomplexes were incubated 2 additional hours with protein A sepharose beads and subjected to washes. After reversing the cross-linking, the DNA was isolated by standart phenol-chloroform-isoamyl extraction procedure. ChIP-Seq libraries were prepared with MicroPlex Library Preparation Kit following the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
20415817
Reads aligned (%)
98.3
Duplicates removed (%)
80.5
Number of peaks
165 (qval < 1E-05)

hg38

Number of total reads
20415817
Reads aligned (%)
99.0
Duplicates removed (%)
78.8
Number of peaks
130 (qval < 1E-05)

Base call quality data from DBCLS SRA