Antigen

Antigen Class

Histone

Antigen

H2AK120Ub

Cell type

Cell type Class

Bone

Cell type

Osteoblasts

MeSH Description

Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.

Sample

source_name

UND_H2Bub1

immortalization type

SV40 large T antigen immortalized

cell type

human Fetal Osteoblasts (hFOB)

protocol

undifferentiated

treatment

DMSO

chip antibody

Cell Signalling, 5546, D11

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIP was performed with 20 min crosslinking in 1 % formaldehyde in PBS. Following chromatin shearing , 1-2µg antibody was used for immunoprecipitation overnight. The immunocomplexes were incubated 2 additional hours with protein A sepharose beads and subjected to washes. After reversing the cross-linking, the DNA was isolated by standart phenol-chloroform-isoamyl extraction procedure. ChIP-Seq libraries were prepared with MicroPlex Library Preparation Kit following the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

18237752

Reads aligned (%)

99.2

Duplicates removed (%)

4.5

Number of peaks

57 (qval < 1E-05)

hg19

Number of total reads

18237752

Reads aligned (%)

99.0

Duplicates removed (%)

4.6

Number of peaks

67 (qval < 1E-05)