Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Bone

Cell type

Osteoblasts

MeSH Description

Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.

Sample

source_name

DIF_JQ1_H3K27ac

immortalization type

SV40 large T antigen immortalized

cell type

human Fetal Osteoblasts (hFOB)

protocol

differentiated for 5 days into osteoblastic lineage

treatment

JQ1

chip antibody

Diagenode, C15410196

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIP was performed with 20 min crosslinking in 1 % formaldehyde in PBS. Following chromatin shearing , 1-2µg antibody was used for immunoprecipitation overnight. The immunocomplexes were incubated 2 additional hours with protein A sepharose beads and subjected to washes. After reversing the cross-linking, the DNA was isolated by standart phenol-chloroform-isoamyl extraction procedure. ChIP-Seq libraries were prepared with MicroPlex Library Preparation Kit following the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

17745978

Reads aligned (%)

99.5

Duplicates removed (%)

12.7

Number of peaks

19990 (qval < 1E-05)

hg19

Number of total reads

17745978

Reads aligned (%)

99.3

Duplicates removed (%)

12.8

Number of peaks

19912 (qval < 1E-05)