GSM2187184: Post-H/S rep 1 Pol II S2P ChIPSeq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Embryonic fibroblast
Cell type
NIH/3T3
Primary Tissue
Embryo
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Post-H/S Pol II S2P_ChIPSeq
cell type
NIH/3T3 Fibroblasts
passages
15-18
chip antibody
Pol II S2P panel (Abcam, catalog# ab103968, lot# 139798-3)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA: Trizol followed by depletion of ribosomal RNA using the ribominus V2 kit (Life technologies) and size selection using the mirVana separation kit (Life technologies), DNA: Phenol-Chloroform Libraries were prepared according to NEB instructions accompanying the following kits: ChIP/CHART-sequencing: NEBNext ChIP-seq library Prep Master MIX set, Short-RNA-sequencing:NEBnext small RNA library construction kit (NEB), RNA-sequencing: NEBNext Ultra directional RNA library kit (NEB)