Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Digestive tract

Cell type

Colon

NA

NA

Sample

source_name

Mouse

tissue

colon

chip antibody

input

sample type

DNA control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to instructions of NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240S/L)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

13773328

Reads aligned (%)

81.8

Duplicates removed (%)

7.9

Number of peaks

1810 (qval < 1E-05)

mm9

Number of total reads

13773328

Reads aligned (%)

81.7

Duplicates removed (%)

8.1

Number of peaks

1768 (qval < 1E-05)