Antigen

Antigen Class

TFs and others

Antigen

SPI1

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

THP1

cell line

THP1

chip antibody

Cell Signaling, 2266s

treatment

BRM014

Sequenced DNA Library

library_name

GSM6706077

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were obtained from sonicated nuclei crosslinked with either 1% formaldehyde and 2 mM disuccinimidyl glutarate (double cross-linking) for SMARCA4, or 1% formaldehyde alone (single cross-linking) for all other targets. Protein-DNA complexes were isolated by immunoprecipitation. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

32645286

Reads aligned (%)

98.7

Duplicates removed (%)

25.4

Number of peaks

73888 (qval < 1E-05)

hg19

Number of total reads

32645286

Reads aligned (%)

98.2

Duplicates removed (%)

25.7

Number of peaks

73527 (qval < 1E-05)