Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEIS1

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
THP1
cell line
THP1
chip antibody
Abcam, ab19867
treatment
DMSO

Sequenced DNA Library

library_name
GSM6706075
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were obtained from sonicated nuclei crosslinked with either 1% formaldehyde and 2 mM disuccinimidyl glutarate (double cross-linking) for SMARCA4, or 1% formaldehyde alone (single cross-linking) for all other targets. Protein-DNA complexes were isolated by immunoprecipitation. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
24937504
Reads aligned (%)
98.8
Duplicates removed (%)
14.3
Number of peaks
6029 (qval < 1E-05)

hg19

Number of total reads
24937504
Reads aligned (%)
97.8
Duplicates removed (%)
14.6
Number of peaks
5867 (qval < 1E-05)

Base call quality data from DBCLS SRA