Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Lung
Cell type
MRC-5
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
OSvKM_PolII
chip antibody
Rpb1 CTD (Cell Signaling 2629S Lot: 3)
cell line
MRC5 fibroblasts
protocol
Oct4, Sox2-VP16, Klf4 and Myc addition
time
5 days
passage
8

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
See ChIP-Endo Protocol.pdf

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
7295649
Reads aligned (%)
82.6
Duplicates removed (%)
2.4
Number of peaks
188 (qval < 1E-05)

hg19

Number of total reads
7295649
Reads aligned (%)
81.9
Duplicates removed (%)
2.4
Number of peaks
163 (qval < 1E-05)

Base call quality data from DBCLS SRA