SRX1813535
GSM2183712: mv411 H3K27ac; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11(Cas9; ENL-FKBP(F36V); ENL-/-)
input sample
mv411_input
treatment
none
growth protocol
RPMI, 10% FBS
sequencing barcode
TGTATGCG
antibody
H3K27ac
antibody manufacturer
abcam
antibody catalog number
ab4729

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
53625145
Reads aligned (%)
98.0
Duplicates removed (%)
15.2
Number of peaks
50391 (qval < 1E-05)

hg19

Number of total reads
53625145
Reads aligned (%)
97.7
Duplicates removed (%)
16.0
Number of peaks
50406 (qval < 1E-05)

Base call quality data from DBCLS SRA