Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
NB-4
Primary Tissue
Blood
Site of Extraction
Bone Marrow
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
NB4
cell line
NB4
cell type
leukemic cells
chip antibody
antibody RUNX1 (Abcam ab23980)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
32313373
Reads aligned (%)
88.6
Duplicates removed (%)
9.6
Number of peaks
34167 (qval < 1E-05)

hg38

Number of total reads
32313373
Reads aligned (%)
89.9
Duplicates removed (%)
8.8
Number of peaks
34196 (qval < 1E-05)

Base call quality data from DBCLS SRA