Antigen

Antigen Class

TFs and others

Antigen

RUNX1

Cell type

Cell type Class

Blood

Cell type

NB-4

Primary Tissue

Blood

Site of Extraction

Bone Marrow

Tissue Diagnosis

Leukemia

Sample

source_name

NB4

cell line

NB4

cell type

leukemic cells

chip antibody

antibody RUNX1 (Abcam ab23980)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

32313373

Reads aligned (%)

98.5

Duplicates removed (%)

8.8

Number of peaks

34196 (qval < 1E-05)

hg19

Number of total reads

32313373

Reads aligned (%)

98.0

Duplicates removed (%)

9.6

Number of peaks

34167 (qval < 1E-05)