GSM2176895: CD4 ACT KAP1 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TRIM28
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD4_ACT_KAP1
tissue
peripheral blood
cell type
CD4+ T lymphocytes
culture condition
3-day culture in activating condition (anti-CD3, anti-CD28, IL-2)
chip antibody
KAP1 (custom rabbit polyclonal)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total inputs and ChIPed samples were prepared for libraries using 10ng of material. Aliquots of the samples were tested in qPCR using the KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems, KK4604) to determine the optimal number of PCR cycles needed to amplify each library without reaching saturation. Libraries were amplified with KAPA HiFi Hotstart Readymix polymerase (KAPA Biosystems, KK2602), size-selected using Ampure XP beads (Beckman Coulter) and always quality checked on Bioanalyser DNA high sensitivity chip (Agilent) and quantified by picogreen (Qubit® 2.0 Fluorometer, Invitrogen). Chromatin was prepared from 10 million cells as previously described (Rowe et al., 2013b).