GSM6670741: MDA-MB-231, SAP30, Bethyl, rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SAP30
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231
cell line
MDA-MB-231
cell type
Breast cancer cells
genotype
Parental
chip antibody
SAP30 (Bethyl Laboratories, #A303-551A)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6670741
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a ultrasonicator (Brandson). ChIP-seq libraries were prepared using the ThruPLEX DNA-seq kit (Takara). 1 ng of DNA was used as starting material for input and ip samples. Libraries were amplified 13 cycles on the thermocycler. Post amplification libraries were gel-purified for size selection at 300-500bp. Libraries were validated using Tapestation (Agilent) and quantified using Qubit Fluorometer (Invitrogen).