Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2C

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 breast cancer cell line
cell line
MCF7
chip antibody
custom anti-MLL3 antibody was provided by Prof. Ali Shilatifard (Stowers Institute, Kansas City, USA and Northwestern University Feinberg School of Medicine, Chicago, USA)
cell type
asynchonous cells
treatment
FOXA1 siRNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq, Schmidt et al, 2009. PMID: 19275951 After amplification, DNA was extracted using QIAgen MinElute gel extraction kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
53327622
Reads aligned (%)
78.4
Duplicates removed (%)
9.8
Number of peaks
14579 (qval < 1E-05)

hg38

Number of total reads
53327622
Reads aligned (%)
79.4
Duplicates removed (%)
9.1
Number of peaks
14683 (qval < 1E-05)

Base call quality data from DBCLS SRA