Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Neural

Cell type

hTERT RPE-1

Primary Tissue

Eye

Tissue Diagnosis

Normal

Sample

source_name

RPE1

treatment

DMSO

cell line

RPE1

cell type

Human retinal pigment epithelial

chip antibody

Active Motif; 613111; lot 34614003; dilution 1:150

Sequenced DNA Library

library_name

GSM6632357

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor For the ChIP-seq library preparation we have followed Illumina's TruSeq Chip Sample Preparation Guide including NextFlex UDIs. have followed Illumina's TruSeq Chip Sample Preparation Guide including NextFlex UDIs.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

7152752

Reads aligned (%)

94.9

Duplicates removed (%)

6.7

Number of peaks

18118 (qval < 1E-05)

hg19

Number of total reads

7152752

Reads aligned (%)

94.4

Duplicates removed (%)

7.2

Number of peaks

18128 (qval < 1E-05)