GSM6617833: 1238 ZEB1 ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ZEB1
Cell type
Cell type Class
Neural
Cell type
Dorsal lateral prefrontal cortex
NA
NA
Attributes by original data submitter
Sample
source_name
DLPFC
disease state
control
cell type
NeuN
tissue
DLPFC
Sex
male
age
63
chip antibody
anti-ZEB1 (Bethyl A301-921A)
Sequenced DNA Library
library_name
GSM6617833
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Post-mortem brain tissue was homogenized and fixed for 10 minutes at room temperature, followed by 5 minutes of quenching with Glycine. Fixed nuclei were isolated by ultracentrifuging 24,000 RPM for 2 hours at 4C. If sorted, nuclei were stained for NeuN/OLIG overnight and sorted the next day. Sorted nuclei were pelleted and resuspended in RIPA for 8 rounds of sonication using the Diagenode Bioruptor Pico. Libraries were prepared by blunting and ligating ChIP DNA fragments to sequencing adapters for amplification with barcoded primers (30 sec at 98°C; [10 sec at 98°C, 30 sec at 65°C, 30 sec at 72°C] x 15 cycles; 5 min at 72°C). Libraries were quantified with Qubit dsDNA HS Assay kit and visualized with Standard Sensitivity NGS Fragment Analysis Kit (Advanced Analytical DNF-473) and Fragment Analyzer 5200 (Agilent).