GSM6617147: CTCF-Trip 4hrs TRE3G-WT-HA 5PhIAA Dox R1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
cell line
cell type
SEM
antibody
CTCF
treatment
Triptolide
Sequenced DNA Library
library_name
GSM6617147
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20million cells were fixed for 5mins with 1% formaldehyde. Covaris truChIP chromatin shearing kit was used to prepare chromatin. Spike-in control was included (Active Motif). Chromatin was incubated overnight with 10ug CTCF antibody (Diagenode). 50ul Protein G dynabeads were added and incubated for 4hrs at 4oC. For HA tagged samples, spike in was not included. Sheared chromatin was incubated for 4hrs with 25ul Anti-HA magnetic beads (Pierce). For all samples, after washing DNA was decrosslinked and eluted in TE+1%SDS, Proteinase K, 400uM NaCl. DNA was purified by phenol cholorform isoamyl alcohol.