Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

H1-hESC

treatment

None

antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27me3 and H3K4me3 antibody. libraries were set up by Berry Genomics BeiJing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

23705308

Reads aligned (%)

93.7

Duplicates removed (%)

32.2

Number of peaks

33101 (qval < 1E-05)

hg19

Number of total reads

23705308

Reads aligned (%)

93.5

Duplicates removed (%)

32.4

Number of peaks

33006 (qval < 1E-05)