GSM2154987: ChIP-Seq ERα plus E2 and Dex; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
breast cancer
cell line
MCF7
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, some samples were fixed for 10 minutes with 1% formaldehyde. Fixation was stopped by adding Glycine (0.125M). Chromatin DNA was sheared to 200–500 bp average in size by sonication. Soluble sheared chromatin was immunoprecipitated with specific antibodies or streptavidin beads overnight at 4°C. After washing and elution, the protein–DNA complex was decross-linked by heating at 65°C overnight. Immunoprecipitated DNA was purified by using iPure Diagenode kit. the libraries were constructed following Illumina's Chip-Seq Sample prep kit.