Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

testes

age

4-week-old

genotype

homozygous

tissue

testes

strain

C57BL/6 (B6) X 129/SvJ hybrid

chip antibody

Mouse anti-Pol II (Abcam, ab817)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Testicular cells were collected by collagenase and trypsin treatment. SimpleChIP Enzymatic Chromatin IP Kit was used to prepare ChIP-seq libraries.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

29120789

Reads aligned (%)

92.1

Duplicates removed (%)

15.7

Number of peaks

713 (qval < 1E-05)

mm9

Number of total reads

29120789

Reads aligned (%)

91.8

Duplicates removed (%)

15.5

Number of peaks

707 (qval < 1E-05)