Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Creb1

Cell type

Cell type Class
Adult
Cell type
Mushroom Bodies
MeSH Description
Prominent lobed neuropils found in ANNELIDA and all ARTHROPODS except crustaceans. They are thought to be involved in olfactory learning and memory.

Attributes by original data submitter

Sample

source_name
mushroom body
strain
CS10
chip antibody
anti-CREB (lab made antibodies)
treatment
naïve

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Heads were collected and homogenized in crosslinking buffer. The nuclei were dissolved in extraction buffer, sonicated to dissociate the individual nuclei, and precipitated with ANTI-FLAG M2 Affinity Gel. The precipitates were rinsed 4 times in extraction buffer, with 5 min nutation at 4℃ between washes, and subjected to ChIP analysis. The purified mushroom body nuclei were sonicated, resulting in fragmentation of DNA ranging from 100 bp to 500 bp. The extracts were centrifuged to remove insoluble materials including the M2 Affinity Gel, and the supernatants were used for immunoprecipitation with specific antibodies. The ChIP DNA samples prepared were used to prepare a library for Miseq, according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina MiSeq

dm3

Number of total reads
5718830
Reads aligned (%)
94.0
Duplicates removed (%)
3.9
Number of peaks
5693 (qval < 1E-05)

dm6

Number of total reads
5718830
Reads aligned (%)
93.7
Duplicates removed (%)
4.3
Number of peaks
5854 (qval < 1E-05)

Base call quality data from DBCLS SRA