Antigen

Antigen Class

TFs and others

Antigen

FOSL1

Cell type

Cell type Class

Others

Cell type

T24

Primary Tissue

Urinary Bladder

Tissue Diagnosis

Carcinoma

Sample

source_name

T24

cell line

T24

cell type

Bladder cancer cell

treatment

Untreated

chip antibody

FRA1 (Cell Signaling 5281)

Sequenced DNA Library

library_name

GSM6588860

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin extraction and chromatin immunoprecipitation was performed following Schubeler Lab Protocol (Weber et al. 2007). ChIP-seq libraries were prepared using Illumina Kits at EMBL's GeneCore Facility & TruSeq DNA Sample prep Kit at Macrogen

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

139591071

Reads aligned (%)

64.6

Duplicates removed (%)

12.0

Number of peaks

22748 (qval < 1E-05)

hg19

Number of total reads

139591071

Reads aligned (%)

64.0

Duplicates removed (%)

13.2

Number of peaks

21945 (qval < 1E-05)