Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Cortex

NA

NA

Sample

source_name

Cortex-Input

strain

B6/129

generation

F1

tissue

Cortex

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Rai1-DNA complexes were isolated with anti-FLAG antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Nextera DNA Library Prep Kit).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

39337346

Reads aligned (%)

97.3

Duplicates removed (%)

6.9

Number of peaks

319 (qval < 1E-05)

mm9

Number of total reads

39337346

Reads aligned (%)

97.1

Duplicates removed (%)

7.6

Number of peaks

327 (qval < 1E-05)