Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
EpiSC
NA
NA

Attributes by original data submitter

Sample

source_name
EpiSCs
tissue
EpiSCs
experiment type
ChIP-seq
antibody
Abcam, ab8580
wnt stimulation
72h CHIR99021

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslink, Lyse, Sonication and Immunoprecipitate Illumina Truseq DNA library protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
57058795
Reads aligned (%)
96.6
Duplicates removed (%)
27.4
Number of peaks
20250 (qval < 1E-05)

mm9

Number of total reads
57058795
Reads aligned (%)
96.4
Duplicates removed (%)
27.4
Number of peaks
20308 (qval < 1E-05)

Base call quality data from DBCLS SRA