Doxycycline-treated BCR-ABL1-transformed primary B cell precursors that carry the empty vector (EV) respective to the DOX-inducible CEBPA vector used in the other experimens. Cells are succesfully transformed cells.
genotype
C57BL/6
vector transduced
MSCV BCR-ABL1-p210; pRetroX-TRE3G; pRetroX-Tet3G
antibody
Anti-gamma H2A.X (phospho S139) (ab2893, Abcam)
treatment
Doxycycline treated
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were enriched for CD11B and dead cells removed using MACS, cells formaldehyde treated and pellets snap frozen. Pellets were resuspended in sonication buffer, sonicated and lysed in RIPA buffer before performing ChIP with the appropriate antibody. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and NEBNEXT Multiplex Oligos for Illumina.