C-MYC expressing primary B cell precursors (D7-9 post transduction)
genotype
53BP1-/- C57BL/6
vector transduced
C-MYC MIGR1
antibody
Anti-gamma H2A.X (phospho S139) (ab2893, Abcam)
treatment
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Dead cells were removed, cells formaldehyde treated and pellets snap frozen. Pellets were resuspended in sonication buffer, sonicated and lysed in RIPA buffer before performing ChIP with the appropriate antibody. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and NEBNEXT Multiplex Oligos for Illumina.