Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E2F1

Cell type

Cell type Class
Blood
Cell type
MM.1S
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

source_name
Multiple Myeloma
cell line
MM1.S
input
MM1S_WCE_MUNSHI
treatment
none
barcode
N/A
antibody
anti E2F1
antibody maker
Cell Signaling
antibody catalog number
Cell Signaling #3742

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and chromatin-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
74738067
Reads aligned (%)
49.9
Duplicates removed (%)
47.0
Number of peaks
8811 (qval < 1E-05)

hg38

Number of total reads
74738067
Reads aligned (%)
51.7
Duplicates removed (%)
45.5
Number of peaks
8821 (qval < 1E-05)

Base call quality data from DBCLS SRA