Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETV1

Cell type

Cell type Class
Digestive tract
Cell type
GIST-T1
NA
NA

Attributes by original data submitter

Sample

source_name
GIST-T1 Human GIST Cells
cell line
GIST-T1
antibody
ETV1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated and sheared. For ETV1 IP, chromatin was IP'd using anti-ETV1 antibody (abcam ab81086) and magnetic protein A/G beads.The immunoprecipate was washed and eluted using standing conditions. RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
58647229
Reads aligned (%)
49.7
Duplicates removed (%)
5.5
Number of peaks
21480 (qval < 1E-05)

hg19

Number of total reads
58647229
Reads aligned (%)
49.3
Duplicates removed (%)
6.8
Number of peaks
21909 (qval < 1E-05)

Base call quality data from DBCLS SRA