GSM6463416: K562 Con LDB1 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
LDB1
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
K562 cells
cell type
erythroleukemia cells
chip antibody
LDB1 (sc-11198)
cell line
K562
genotype
control
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6463416
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were digested with 100 units of MNase at 37°C for 15 min. Mono or di-nucleosomes sized chromatin was incubated with antibodies for 3 h at 4°C and recovered with protein G or A agarose beads. ChIP DNA (10 ng for K27ac, Input, 0.5 ng for GATA1, TAL1, LDB1) was processed using NEBNext ChIP-seq library (New England Biolabs) with manufacturer's instructions. The ends of ChIP DNA were repaired by NEBNext Ultra II End Prep enzyme mix and ligated with NEBNext adaptors. Adaptor-ligated DNA was selected at 200 bp size using NEBNext sample purification bead and amplified using the adaptor primers. Amplification cycles were 7 cycles for H3K27ac and input and 12 cycles for GATA1, TAL1 and LDB1. The fragments are purified using NEBNext sample purification beads.